Quantitative Analysis of Xenograft Tumor Biomarkers

A xenograft tumor biopsy was examined using antibodies to Ki-67 (ab15580, 1/100, Abcam) and SLC7A11 (DF12509, 1/100, Affinity) as described in our previous studies [27 (link), 28 (link)]. The staining outcomes were measured utilizing Image-Pro Plus 6.0 (Media Cybernetics, USA) and presented as the proportion of cells exhibiting positivity (for Ki-67) or the average intensity (for SLC7A11). Cell apoptosis in xenograft tumor tissues was detected using the CF488 (Green) Tunel Cell Apoptosis Detection Kit (Servicebio, Wuhan, China) following the guidelines provided by the manufacturer.

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Dong F.L., Xu Z.Z., Wang Y.Q., Li T., Wang X, & Li J. (2024). Exosome-derived circUPF2 enhances resistance to targeted therapy by redeploying ferroptosis sensitivity in hepatocellular carcinoma. Journal of Nanobiotechnology, 22, 298.

Publication 2024

ab15580 DF12509 Image-Pro Plus 6.0 CF488 (Green) Tunel Cell Apoptosis Detection Kit

Corresponding Organization :

Other organizations : Shandong First Medical University, Shandong Provincial QianFoShan Hospital

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Variable analysis

independent variables

Antibody used for Ki-67 staining (ab15580, 1/100, Abcam)
Antibody used for SLC7A11 staining (DF12509, 1/100, Affinity)

dependent variables

Proportion of cells exhibiting positivity for Ki-67
Average intensity of SLC7A11 staining
Apoptosis in xenograft tumor tissues (measured using CF488 (Green) Tunel Cell Apoptosis Detection Kit)

control variables

Xenograft tumor biopsy
Staining procedure described in previous studies [27, 28]
Image-Pro Plus 6.0 software for measurement

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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