DP were captured in Ohio, USA and maintained in culture for many years. DP growth conditions were previously described [19 ]. DNA isolation from whole mite extract is detailed in the Supplemental Material. Explicit methods regarding genome assembly and protein prediction are also described in the Supplemental Material. All original sequence data used herein are deposited to Genbank under Bioproject PRJNA395246.
All multiple sequence alignments were generated using muscle within CLC Genomics Workbench.
Several genomes from Acari suborder, which includes mites and ticks, have recently been published including D. farinae [5 (link)], scabies mites Sarcoptes scabiei (SS) [20 (link)–22 ], the honey bee mite Tropilaelaps mercedesae [23 ], the spider mite Tetranychus urticae [24 (link)], which is preyed upon by the mite Metaseiulus occidentalis [25 (link)], Ixodes scapularis [26 ,27 (link)], and Varroa destructor [28 (link)]. Four mite genomes from the order Oribatida have also been sequenced: Achipteria coleoptrata, Platynothrus peltifer, Steganacarus magnus, and Hypochthonius rufulus [29 (link)]. Genomic data for these species was downloaded from GenBank (Table 1).