Human Hepatocellular Carcinoma Cell Lines

The human HCC cell lines, HEP3B217, HUH7, HEPG2, SNU398, SNU878, HUH6, SKHEP1 and PLCPRF5, were provided by Erasmus University (Rotterdam, Netherlands). MHCC97H were provided by the Liver Cancer Institute of Zhongshan Hospital (Shanghai, China). These cells were maintained in Dulbecco's modified Eagle's medium (DMEM) (Gibco, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS) (Gibco) and 1% penicillin/streptomycin (BasalMedia), incubated at 37 °C in humidified atmosphere with 5% CO2. Mycoplasma contamination was excluded via a PCR-based method. The information of TP53 mutation status of cell lines used in this study was achieved from our previous publication 54 (link). The identities of all the cell lines were confirmed by short tandem repeat (STR) profiling. Volasertib (S2235) and GSK461364 (S2193) were purchased from Selleck Chemicals and dissolved in dimethyl sulfoxide (DMSO) using a storage concentration of 10 mM.

Free full text: Click here

Yang C., Guo Y., Qian R., Huang Y., Zhang L., Wang J., Huang X., Liu Z., Qin W., Wang C., Chen H., Ma X, & Zhang D. (2021). Mapping the landscape of synthetic lethal interactions in liver cancer. Theranostics, 11(18), 9038-9053.

Publication 2021

DMEM penicillin/streptomycin Volasertib (S2235) GSK461364

Atmosphere Cancer of the liver Cell lines Cells Dimethyl sulfoxide Fetal bovine serum Gsk461364 Human Mutation Mycoplasma Penicillin Short tandem repeat Streptomycin Tp53 Volasertib

Corresponding Organization :

Other organizations : Zhejiang University, Shanghai Jiao Tong University, Shanghai Cancer Institute, State Key Laboratory of Oncogene and Related Genes, Renji Hospital, Fujian Medical University, Huazhong University of Science and Technology, Tongji Hospital

Top 5 similar protocols

Variable analysis

independent variables

Volasertib (S2235)
GSK461364 (S2193)

dependent variables

Cell growth/proliferation
Cell viability

control variables

Cell culture conditions (Dulbecco's modified Eagle's medium (DMEM), 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, 37 °C, 5% CO2, humidified atmosphere)
Mycoplasma contamination (excluded via a PCR-based method)
Cell line identity (confirmed by short tandem repeat (STR) profiling)

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!