NanoSIMS Mass Spectrometry Imaging

Data were acquired with the prototype (4 detectors) and a second-generation instrument (NanoSIMS 50L, Cameca: 7 detectors). Quantitative mass images were analysed with “Open MIMS” software, a plug-in to ImageJ (http://www.nrims.harvard.edu/software.php). Stable isotopes were obtained from Cambridge Isotopes, Inc. (¹⁵N-thymidine, ¹³C-thymidine) and Sigma-Aldrich, Inc. (¹³C-palmitate).
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Steinhauser M.L., Bailey A., Senyo S.E., Guillermier C., Perlstein T.S., Gould A.P., Lee R.T, & Lechene C.P. (2012). Quantitating subcellular metabolism with multi-isotope imaging mass spectrometry. Nature, 481(7382), 516-519.

Publication 2011

Isotopes Palmitate Thymidine

Corresponding Organization :

Other organizations : Brigham and Women's Hospital, Medical Research Council, Harvard University, Harvard Stem Cell Institute

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Protocol cited in 16 other protocols

Variable analysis

independent variables

Prototype (4 detectors)
Second-generation instrument (NanoSIMS 50L, Cameca: 7 detectors)

dependent variables

Quantitative mass images

control variables

Stable isotopes obtained from Cambridge Isotopes, Inc. (15N-thymidine, 13C-thymidine) and Sigma-Aldrich, Inc. (13C-palmitate)

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