Microarray data from our previous study [26 (link)] using the hippocampus of 12- and 52-week-old mice were re-analyzed. The microarray data were deposited into the GEO database (accession number GSE144459). Total RNA was isolated from the frozen hippocampus using the RNeasy Mini Kit (QIAGEN, #74104). A NanoDrop1000 device (Thermo Fisher Scientific) was used to measure the concentrations of RNA. An Agilent 2100 Bioanalyzer (Agilent) was used to measure the RNA integrity number (RIN). All of the samples used for the microarray analysis satisfied the following criteria: A260/A280≥1.8, A260/A230≥2.0, and RIN≥7.0. The RIN of each sample is shown in Supplementary Table 1 .
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Hippocampal Gene Expression Analysis
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Corresponding Organization : Ehime University
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Variable analysis
independent variables
- Age of mice (12-week-old vs. 52-week-old)
- Gene expression in the hippocampus
- RNA quality: A260/A280 ≥ 1.8, A260/A230 ≥ 2.0, and RIN ≥ 7.0
- Tissue source: Hippocampus
- Positive control: Not mentioned
- Negative control: Not mentioned
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