Cancer Cell Line Maintenance and Storage

ME180 cells (human cervical cancer), Ishikawa cells (human endometrial cancer), and ID-8 cells (mouse ovarian cancer) were purchased from the American Type Culture Collection. The cell lines were passaged in our laboratory soon after they were received from the cell bank, before being divided and stored in liquid nitrogen vessels. Each experiment was carried out using thawed cells without further authentication. The cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal calf serum, as reported previously^{12 (link)}.

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Shimura K., Mabuchi S., Komura N., Yokoi E., Kozasa K., Sasano T., Kawano M., Matsumoto Y., Watabe T., Kodama M., Hashimoto K., Sawada K., Hatazawa J, & Kimura T. (2021). Prognostic significance of bone marrow FDG uptake in patients with gynecological cancer. Scientific Reports, 11, 2257.

Publication 2021

ME180 cells Ishikawa cells ID-8 cells

Cell lines Cells Cervical cancer Eagle Endometrial cancer Fetal calf serum Human Mouse Nitrogen Ovarian cancer Vessels

Corresponding Organization :

Other organizations : Osaka University, The University of Texas MD Anderson Cancer Center

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Variable analysis

independent variables

Cell lines used: ME180 cells (human cervical cancer), Ishikawa cells (human endometrial cancer), and ID-8 cells (mouse ovarian cancer)

dependent variables

Not explicitly mentioned

control variables

Cells were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum
Experiments were carried out using thawed cells without further authentication

controls

No positive or negative controls were explicitly mentioned in the provided information

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