Mica coverslips were prepared by coupling a mica sheet to a quartz coverslip with optical glue as described elsewhere 58 (link) . Briefly, coverslips were cleaned with 2% detergent (Hellmanex III, Hellma Analytics) and ethanol before gluing previously cut mica leaflets (5 mm x 5 mm, 1872-CA, SPI) on top of them with a low viscosity optical adhesive (NOA60, Norland products). After curing the adhesive with one hour exposure to UV light, another coverslip was glued on top of the mica with a high-viscosity optical adhesive (NOA63, Norland products), followed by another round of UV exposure. Coverslips were separated to expose a freshly cleaved mica surface just before performing SB experiments.
Protocol detail
Preparation of Mica-Coated Coverslips
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Corresponding Organization :
Other organizations : Howard Hughes Medical Institute, Rockefeller University
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Variable analysis
independent variables
- Mica sheet coupling to quartz coverslip with optical glue
- Mica leaflet size (5 mm x 5 mm)
- Optical adhesive type (low viscosity NOA60, high viscosity NOA63)
- Freshly cleaved mica surface
- Detergent (Hellmanex III) and ethanol used for cleaning coverslips
- UV light exposure duration (1 hour) for curing adhesives
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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