Zanamivir (Acme Bioscience) was dissolved in Opti-MEM at a concentration of 50 mM and stored at −80°C. Monoclonal anti-HPIV3 HN antibodies were custom elicited in rats (Aldevron) using eGFP-HN complementary DNA, diluted in Dulbecco’s PBS (DPBS) to 100 μg/ml, and kept at 4°C. PIA174 antibody fragment (Fab) and full antibody (Ab) were purchased from Creative Biolabs. PA3/F4 was purified by Rockland Immunochemicals Inc. and was originally generated as described in (46 (link)).
Protocol detail
Monoclonal Antibodies for HPIV3 Detection
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Corresponding Organization : City College of New York
Other organizations : University of Washington, Fred Hutch Cancer Center
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Variable analysis
independent variables
- Zanamivir (Acme Bioscience) concentration
- Monoclonal anti-HPIV3 HN antibodies concentration
- PIA174 antibody fragment (Fab) and full antibody (Ab)
- PA3/F4 purification
- Not explicitly mentioned
- Zanamivir storage temperature (-80°C)
- Monoclonal anti-HPIV3 HN antibodies storage temperature (4°C)
- Diluent for monoclonal anti-HPIV3 HN antibodies (Dulbecco's PBS)
- Not specified
- Not specified
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