The MDA-MB-231 breast cancer cell line was purchased from ATCC (Manassas, VA) and transfected with red fluorescent protein (RFP), as described previously30 (link). Cells were maintained in RPMI 1640 with 10% heat-inactivated fetal bovine serum and 25 IU/ml penicillin and streptomycin (Gibco, Life Technologies). For seeding into the ex vivo hepatic microphysiological system, cells were trypsinized, centrifuged and resuspended in hepatocyte maintenance medium (detailed below).
Protocol detail
Maintaining RFP-transfected MDA-MB-231 Cells
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Breast cancer
Cells
Fetal bovine serum
Hepatocyte
Mda mb 231 cell line
Microphysiological system
Penicillin
Red fluorescent protein
Streptomycin
Corresponding Organization : VA Pittsburgh Healthcare System
Other organizations : Massachusetts Institute of Technology, UPMC Hillman Cancer Center
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Variable analysis
independent variables
- Transfection of MDA-MB-231 breast cancer cells with red fluorescent protein (RFP)
- Not explicitly mentioned
- Cell culture conditions (RPMI 1640 medium with 10% heat-inactivated fetal bovine serum and 25 IU/ml penicillin and streptomycin)
- Cell preparation method (trypsinization, centrifugation, and resuspension in hepatocyte maintenance medium) for seeding into the ex vivo hepatic microphysiological system
- No positive or negative controls were explicitly mentioned in the provided information.
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