Mice Genetics for Cytokine Signaling

Eight- to 12-week-old male mice on the C57BL/6 genetic background were
used in all experiments. IL-1α-deficient mice and IL-1β-deficient
mice were a kind gift of Yoichiro Iwakura (University of Tokyo, Japan) and were
generated as previously described^{33 (link)}. Wt C57BL/6 mice, TNF-deficient mice
(B6.129S-^Tnftm1Gkl/J), IL-1R-deficient mice
(B6.129S7-Il1r1^tm1Imx/J) were obtained from Jackson Laboratories
(Bar Harbor, ME). The IL-1R-deficent mice and TNF-deficient mice were crossed to
generate mice deficient in both IL-1R1 and TNF (i.e.,
IL-1R/TNF-deficient mice). The group sizes for each mouse strain included at
least 6 mice/group and a group of 4-5 wt mice were included in every experiment
ensure reproducible results prior to combining data from different iterations.
All mice were bred and maintained under specific pathogen-free conditions at an
American Association for the Accreditation of Laboratory Animal Care
(AAALAC)-accredited animal facility at Johns Hopkins University and were housed
according to procedures described in the Guide for the Care and Use of
Laboratory Animals.