Radioligand Binding Assay for Nicotinic Receptors

All IP experiments were performed using IPN tissue obtained from prepubescent mice and rats (17–19 days of age) following decapitation. The protocols for solubilizing, isolating and labelling the receptors with [³H]-epibatidine were as published previously (David et al., 2010 (link); Scholze et al., 2012 (link)). Here, we used standardized Pansorbin cells (catalog number 507861; Calbiochem, Merck-Millipore, Darmstadt, Germany) for IP and the bicinchoninic acid protein assay reagent kit (Thermo Scientific Pierce, Rockford, IL, USA) for protein quantification.

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Beiranvand F., Zlabinger C., Orr-Urtreger A., Ristl R., Huck S, & Scholze P. (2014). Nicotinic acetylcholine receptors control acetylcholine and noradrenaline release in the rodent habenulo-interpeduncular complex. British Journal of Pharmacology, 171(23), 5209-5224.

Publication 2014

Pansorbin cells bicinchoninic acid protein assay reagent kit

Assay Bicinchoninic acid Cells Decapitation Epibatidine Mice Protein Rats Tissue

Corresponding Organization :

Other organizations : Medical University of Vienna, Tel Aviv Sourasky Medical Center, Tel Aviv University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Receptor binding with [3H]-epibatidine

control variables

Age of mice and rats (17-19 days)
Use of IPN tissue from prepubescent animals
Standardized Pansorbin cells for IP
Bicinchoninic acid protein assay reagent kit for protein quantification

controls

Positive control: Not specified
Negative control: Not specified

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