Melting points were recorded using a digital Gallenkamp (SANYO; Loughborough, UK) apparatus. FTIR spectra were recorded using PerkinElmer spectrophotometer (PerkinElmer Inc., Waltham, MA, USA), using potassium bromide (KBr) pellet technique. 1 (link)H NMR and 13 (link)C NMR spectra were determined in CDCl3 solution at 300 MHz on Bruker AM-300 spectrometer (Bruker AXS Inc., Madison, WI, USA). The breast carcinoma cell line (MCF-7) was obtained from Sigma-Aldrich Co. (St Louis, MO, USA). Mass spectra were performed on an Agilent 6460 Series Triple Quadrupole instrument (Agilent Technologies, Santa Clara, CA, USA). The ionization was achieved by electrospray ionization in the positive ion mode (ESI+). The capillary voltage was set to 4.0 kV. The source temperature was 120°C, and the desolvation temperature was 350°C. Nitrogen was used as a desolvation gas (flow 600 L/hour). Elemental analysis (C, H) was carried out on a Flash 2000 series elemental analyzer with TCD detector system (PerkinElmer Inc., Waltham, MA, USA) and results are with ±0.3%.
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Characterization of Synthetic Compounds
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Variable analysis
independent variables
- None explicitly mentioned
- Melting points
- FTIR spectra
- 1H NMR and 13C NMR spectra
- Mass spectra
- Elemental analysis (C, H)
- Digital Gallenkamp (SANYO; Loughborough, UK) apparatus for melting point determination
- PerkinElmer spectrophotometer (PerkinElmer Inc., Waltham, MA, USA) for FTIR spectra
- Bruker AM-300 spectrometer (Bruker AXS Inc., Madison, WI, USA) for NMR spectra
- Agilent 6460 Series Triple Quadrupole instrument (Agilent Technologies, Santa Clara, CA, USA) for mass spectra
- Flash 2000 series elemental analyzer with TCD detector system (PerkinElmer Inc., Waltham, MA, USA) for elemental analysis
- CDCl3 solvent used for NMR spectra
- Electrospray ionization in positive ion mode (ESI+) for mass spectra
- Capillary voltage of 4.0 kV, source temperature of 120°C, and desolvation temperature of 350°C for mass spectra
- Nitrogen used as desolvation gas (flow 600 L/hour) for mass spectra
- MCF-7 breast carcinoma cell line obtained from Sigma-Aldrich Co. (St Louis, MO, USA)
- Positive control: None mentioned
- Negative control: None mentioned
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