The 1-butanol/methanol (1:1 v/v) method was compared with two other single-phase methods; a one-phase chloroform/methanol method we have previously described [6 (link)], as well as a single-phase 1-butanol/methanol (3:1 v/v) modified from the method reported by Löfgren et al. [23 (link)]. All methods require only a single extraction.
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