Proteomics analysis of EV samples was processed on a liquid chromatography electrospray ionisation tandem mass spectrometer (LC-ESI MS/MS, Shanghai Applied Protein Technology Co., Ltd., Shanghai, China) as previously reported [37 (link)]. After EVs (equivalent to 30 μg total proteins) were treated with trypsin digestion, the peptides were collected and loaded onto the Easy-nLC1000 system equipped with the orbitrap Q Exactive MS (Thermo). The LC-ESI MS/MS full-scan signals were acquired within the range of the precursor ion from 300 to 1800 m/z, and original files were imported into Mascot software (version 2.2, Matrix Science, London, UK) for protein candidate identification and characterisation. The reference database was uniprot_Homo_sapiens_173343_20191014.fasta (173,343 protein sequences, download date of 14 October 2019).
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