C57/BL6 12–14-week-old male mice were housed under a 12 h light/12 h dark cycle regimen for two weeks with food available during the night. Two-third partial hepatectomies were performed as described (21–23 (link)). Three pools of three mice were processed in one batch between ZT01.5 and ZT02.5, with three mice operated every 20 minutes. The livers of the three mice were pooled for each timepoint. ChIPs were performed as described (24 (link)). The following antibodies were used: anti-RPC4 (CS681) (2 (link)), anti-H3K4me3 (Abcam, ab8580) and anti-RPB2 (Santa Cruz Biotechnology, sc-673-18). It should be noted that the anti-H3K4me3 antibody used scored as the best ENCODE-validated anti-H3K4me3 antibody but is 60–66% specific for H3K4me3, with crossreaction to H3K4me2 and very weak crossreaction with H3K4me1 (25 (link)).
Free full text: Click here
