Antibodies for Retinal Ganglion Cell Labeling

Rabbit polyclonal antibody against the green fluorescent protein (GFP) conjugated with AlexaFluor 488 was purchased from Molecular Probes (Eugene, OR, USA; Catalog No. A21311). This antibody was raised against GFP isolated directly from Aequorea victoria and has been previously characterized by immunocytochemistry in granule cells [46 (link)], olfactory sensory neurons [47 (link)], and hippocampal neurons that express GFP [48 (link)]. An anti-RBPMS (RNA-binding protein with multiple splicing) antibody was purchased from PhosphoSolutions (Aurora, CO, USA; Catalog #: 1832-RBPMS). This polyclonal antibody was raised in guinea pigs against a synthetic peptide corresponding to amino acid residues from the N-terminal region of the rat RBPMS sequence conjugated to KLH. This antibody has been characterized by Western blotting and verified with immunocytochemistry on mammalian retinas and demonstrated to be a specific pan-RGC marker which labels all RGCs but not any other cells in the retina [49 (link),50 (link),51 (link)]. The secondary antibodies were purchased from Jackson Immune Research Laboratories (West Grove, PA, USA).

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Yang N., Young B.K., Wang P, & Tian N. (2020). The Susceptibility of Retinal Ganglion Cells to Optic Nerve Injury is Type Specific. Cells, 9(3), 677.

Publication 2020

AlexaFluor 488 anti-RBPMS

Amino acid Antibodies Antibody Cells Granule Green fluorescent protein Guinea pigs Immunocytochemistry Mammalian Molecular probes Neurons Olfactory sensory neurons Peptide Rabbit Rbpms Retina

Corresponding Organization : University of Utah

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Variable analysis

independent variables

Rabbit polyclonal antibody against the green fluorescent protein (GFP) conjugated with AlexaFluor 488
Anti-RBPMS (RNA-binding protein with multiple splicing) antibody

dependent variables

Immunocytochemistry in granule cells
Immunocytochemistry in olfactory sensory neurons
Immunocytochemistry in hippocampal neurons that express GFP
Western blotting and immunocytochemistry on mammalian retinas

control variables

The rabbit polyclonal antibody against GFP has been previously characterized by immunocytochemistry in various cell types
The anti-RBPMS antibody has been verified to be a specific pan-RGC marker which labels all RGCs but not any other cells in the retina

positive controls

Immunocytochemistry on granule cells, olfactory sensory neurons, and hippocampal neurons that express GFP to characterize the rabbit polyclonal antibody against GFP
Western blotting and immunocytochemistry on mammalian retinas to verify the anti-RBPMS antibody as a specific pan-RGC marker

negative controls

The anti-RBPMS antibody has been demonstrated to label all RGCs but not any other cells in the retina

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