Characterization of Prostate Cancer Cell Lines

PC3 cells were a generous gift from Dr. Carrie Rinker-Schaeffer. TRAMPC2, DU145, and LNCaP cells were purchased from ATCC, and ARCaP_M obtained from Novicure Biotechnology. PC3 and ARCaP_M cell line variants were generated as previously described [9 (link)]. Antibodies used for Western blot analysis or multiplexed quantum dot labeling (mQDL) were: anti-FYN antibody (Cell Signaling #4023 or Santa Cruz #sc16), phospho-MET (#3126) and total MET (#4560) obtained from Cell Signaling. Anti-CD44 (sc-7297); anti-CD56 (sc-7326); anti-CHGA (sc-13090), anti-SYP (sc-17750), all obtained from Santa Cruz. Other common reagents for mQDL were used as described previously [39 , 40 (link)]. Recombinant human HGF was purchased from Calbiochem (Millipore).

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Gururajan M., Cavassani K.A., Sievert M., Duan P., Lichterman J., Huang J.M., Smith B., You S., Nandana S., Chu G.C., Mink S., Josson S., Liu C., Morello M., Jones L.W., Kim J., Freeman M.R., Bhowmick N., Zhau H.E., Chung L.W, & Posadas E.M. (2015). SRC family kinase FYN promotes the neuroendocrine phenotype and visceral metastasis in advanced prostate cancer. Oncotarget, 6(42), 44072-44083.

Publication 2015

TRAMPC2 DU145 LNCaP

Anti antibody Antibodies Cd44 Cell line Cells Human Western blot analysis

Corresponding Organization : Cedars-Sinai Medical Center

Other organizations : Huntington Medical Research Institutes

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