Wild type and mutant viruses expressing HEK-293T cells were cultured in DMEM with 5% bovine growth serum (Gibco, Carlsbad, CA). De-identified PBMCs were provided from the Human Immunology Core at the University of Pennsylvania. The core maintains an IRB approved protocol in which the declaration of Helsinki protocols was followed and each donor gave written informed consent. PBMCs were maintained in RPMI with 10% fetal bovine serum (FBS) (Hyclone, South Logan, Utah). A10 (EBNA3C), and S12 (LMP1) hybridomas were described previously [32 (link), 33 (link)]. BZLF1 antibody was provided by Martin Rowe (University of Birmingham, UK) [34 (link)]. EBNA1 antibody was purchased from Advanced Biotechnologies, Inc., Columbia, MD. β-Catenin antibody was procured from Cell Signaling, Inc. Beverly, MA. Rabbit polyclonal RBP-Jk antibody was provided by Elliott Kieff (Harvard Medical School, Boston, MA). Nm23-H1 antibody was procured from Seikagaku Corp (Tokyo, Japan). The p-ERK (E-4), p-JNK (G-7), p-P38 (D-8) antibodies were purchased from Santa Cruz Biotechnology, Inc (Santa Cruz, CA), and the GAPDH antibody was obtained from US-Biological Corp. (Swampscott, MA).
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