Astrocyte Differentiation from hESCs

Plates were coated with growth factor-reduced Matrigel (BD Biosciences). NPC- and Olig2PC-Astro cell lines, both derived from human embryonic stem cells, were established and provided by Dr. Wenbin Deng (Sun Yat-sen University) 5 (link). NPC- and Olig2PC-Astros were cultured on separate plates in serum-free DMEM/F12 medium (Invitrogen) containing 1× N2 (Invitrogen), 1× B27 without retinoic acid (Invitrogen), 10 ng/ml BMP4 (Peprotech), and 20 ng/ml bFGF (Millipore). The medium was changed every 2 days until transplantation.

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Wang J., Jiang P., Deng W., Sun Y, & Liu Y. (2022). Grafted human ESC-derived astroglia repair spinal cord injury via activation of host anti-inflammatory microglia in the lesion area. Theranostics, 12(9), 4288-4309.

Publication 2022

Matrigel DMEM/F12 medium B27 without retinoic acid BMP4 bFGF

Bmp4 Cell lines Growth factor Human embryonic stem cells Matrigel Retinoic acid Serum Transplantation

Corresponding Organization :

Other organizations : Soochow University, Second Affiliated Hospital of Soochow University, Rutgers, The State University of New Jersey, Sun Yat-sen University, Tongji University, Nantong University, Shanghai Tenth People's Hospital

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Variable analysis

independent variables

Culture of NPC- and Olig2PC-Astro cell lines
Culture medium composition (serum-free DMEM/F12, N2, B27 without retinoic acid, BMP4, bFGF)

dependent variables

Not explicitly mentioned

control variables

Plates coated with growth factor-reduced Matrigel
Culture medium changed every 2 days until transplantation

controls

No positive or negative controls explicitly mentioned

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