Quantifying Fecal Amino Acids in Probiotic Studies
Total free amino acids (FAAs) in water-soluble extracts of fecal media were determined using a Biochrom 30 series Amino Acid Analyzer (Biochrom Ltd., Cambridge Science Park, Waterbeach Cambridge, UK), equipped with a sodium cation-exchange column (20 by 0.46 cm, inner diameter), as previously described [29 (link)]. The analysis was performed after 24 h of probiotic growth in fecal media obtained from pooled and sterilized feces of healthy subjects (HC) or chronic kidney disease patients (CKD).
Vacca M., Celano G., Lenucci M.S., Fontana S., la Forgia F.M., Minervini F., Scarano A., Santino A., Dalfino G., Gesualdo L, & De Angelis M. (2021). In Vitro Selection of Probiotics, Prebiotics, and Antioxidants to Develop an Innovative Synbiotic (NatuREN G) and Testing Its Effect in Reducing Uremic Toxins in Fecal Batches from CKD Patients. Microorganisms, 9(6), 1316.
Probiotic growth in fecal media obtained from pooled and sterilized feces of healthy subjects (HC) or chronic kidney disease patients (CKD)
dependent variables
Total free amino acids (FAAs) in water-soluble extracts of fecal media
control variables
Fecal media obtained from pooled and sterilized feces of healthy subjects (HC) or chronic kidney disease patients (CKD)
Probiotic growth for 24 hours
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