Orthotopic Glioma Mouse Model for Anti-Cancer Therapy

For in vivo experiments, 5 × 10⁵ glioma cells were orthotopically transplanted (Stereotaxic coordinate: X(AP) = 1.0 mm, Y(ML) = 2.0 mm, Z(DV) = −3 mm) in Nude mice (Charles River). For bioluminescence imaging, cells were labeled with the lentiviral-based reporter co-expressing RFP and luciferase (SBI). Oral drugs were delivered to xenografted mice once daily by gavage from d2 to d16. Animals were closely monitored for tumor growth, and euthanized when neurologic signs of disease develop. In addition, subsets were sacrificed at appropriate intervals and tissue sections microscopically examined for early evidence of tumor formation. Methods for the analysis of tumor xenograft for morphology, size, proliferation, and differentiation have previously been reported [28 (link),29 (link),30 (link)].