Prostate Cancer Cell Line Authentication

RWPE-1, PC3, DU145, VCaP, 22Rv1 and LNCaP were obtained from American Type Culture Collection (ATCC), that are authenticated by Short Tandem Repeat Profiling and used within 5–10 passages and replenished from stocks. C4-2B is described earlier². All cell lines were incubated in a humidified atmosphere of 5% CO₂ at 37 °C and cultured as described earlier^{2 (link)}. Cultures are routinely tested for Mycoplasma contamination using the MycoAlert™ PLUS Mycoplasma Detection Kit from Lonza and by PCR based screening. HOXB13 F-9 monoclonal antibody (Cat #SC-28333) was purchased from Santa Cruz and HOXB13 rabbit polyclonal antibodies were purchased from Genetex (GTX129245). HSPB8 (Abcam Cat# Ab79784), Actin antibodies (Sigma-Aldrich Cat# A2228), were purchased from Abcam. AR (F39.4.1) antibody was purchased from Biogenex. siRNAs were purchased from multiple sources. Source for all siRNA and sequences are provided in the Supporting data section. GFP-HSPB8 expression vector was synthesized and sequenced by Genscript. Prostate cancer cells were transfected with siRNAs or plasmids using LONZA nucleofector kit.