SUM159 cells were treated with NExT or PLGA NPs (100 µg/ml) loaded with coumarin-6 for 5, 15, and 30 min. The MDA-MB-468 cell line was cultured in the presence/absence of IFNγ (100 ng/ml) for 24 h and treated with NExT or NNaT (100 µg/ml) loaded with coumarin-6. For the rescue experiment, MDA-MB-468 cells (with/without IFNγ, 100 ng/ml) were treated with the anti-PDL1 antibody atezolizumab (10 µg/ml) for 24 h and coumarin-6-loaded NExT or PLGA NPs (100 µg/ml) for 15 min. PDL1-transduced or wild-type Namalwa and Nalm7 cells were treated with NExT or PLGA NPs (100 µg/ml) loaded with coumarin-6 for 5 and 15 min, and 30 and 60 min, respectively. The percentage of cells positive for coumarin-6 fluorescence was detected in the FITC channel by the FACS Verse cytometer and analyzed with FlowJo.
Other organizations :
Complejo Hospitalario de Jaén, Instituto de Investigación Biosanitaria de Granada, Universidad de Granada, Pfizer-University of Granada-Junta de Andalucía Centre for Genomics and Oncological Research, Consejo Superior de Investigaciones Científicas, Leiden University Medical Center
Treatment with NExT or PLGA NPs (100 µg/ml) loaded with coumarin-6
Treatment with NExT or NNaT (100 µg/ml) loaded with coumarin-6
Treatment with the anti-PDL1 antibody atezolizumab (10 µg/ml)
PDL1 transduction in Namalwa and Nalm7 cells
dependent variables
Percentage of cells positive for coumarin-6 fluorescence detected in the FITC channel by the FACS Verse cytometer
control variables
Incubation time (5, 15, and 30 min; 15 min; 5 and 15 min; 30 and 60 min)
Cell lines (SUM159, MDA-MB-468, Namalwa, Nalm7)
Presence/absence of IFNγ (100 ng/ml) treatment in MDA-MB-468 cells
controls
Positive control: MDA-MB-468 cells treated with IFNγ (100 ng/ml) for 24 h
Negative control: MDA-MB-468 cells without IFNγ treatment
Annotations
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