IHC Evaluation of Rab27A in NSCLC

Immunohistochemical (IHC) assays were performed as described previously^{39 (link)}. Tissue microarrays of 180 NSCLC tissues and matched adjacent normal tissues (90 pairs of adenocarcinomas: HLugA180Su02; 90 pairs of squamous cell carcinoma: HLugS180Su01; Outdo Biotech, Shanghai, China) were used to evaluate Rab27A protein expression. Tissue microarrays (ZL-LugA961) were obtained from Zhuolibiotech (Shanghai, China) to detect Rab27A and HSPA5 protein levels.

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Zeng Y., Zhao J., Wu Z., Huang Y., Wang A., Zhu J., Xu M., Zhang W., Zhang X., Li J., Huang J.A, & Liu Z. (2024). Targeting TYK2 alleviates Rab27A-induced malignant progression of non-small cell lung cancer via disrupting IFNα-TYK2-STAT-HSPA5 axis. NPJ Precision Oncology, 8, 74.

Publication 2024

HLugA180Su02 HLugS180Su01

Corresponding Organization :

Other organizations : Soochow University, First Affiliated Hospital of Soochow University, Second Affiliated Hospital of Soochow University

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Variable analysis

independent variables

Rab27A protein expression
Rab27A protein levels
HSPA5 protein levels

dependent variables

NSCLC tissues and matched adjacent normal tissues

control variables

Tissue microarrays of 180 NSCLC tissues and matched adjacent normal tissues (90 pairs of adenocarcinomas: HLugA180Su02; 90 pairs of squamous cell carcinoma: HLugS180Su01)
Tissue microarrays (ZL-LugA961)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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