Quantitative Analysis of SCFAs

All short-chain fatty acids standards ((acetic acid-AA (C2), propionic acid- PA (C3), butyric acid-BA (C4), isobutyric acid-IBA (C4), valeric acid- VA (C5), isovaleric acid-IVA (C5), caproic acid—CA (C6), isocaproic acid ICA (C6)) and short-chain fatty acid isotope-labeled standards (acetic acid-13C2, propionic acid-D6, butyric acid-13C2, isobutyric acid-D7, valeric acid-D9), pyridine anhydrous, 2-nitro phenylhydrazine (3NPH·HCl), N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC·HCl)) were acquired from Sigma-Aldrich (St. Louis, MO, USA). All SCFAs stock solutions were prepared in 50% acetonitrile and stored at −20 °C. LC-MS grade acetonitrile, HPLC-grade acetonitrile, HPLC-grade methanol, and formic acid were obtained from J.T. Baker. Ultra-pure water (Mili-Q water) was produced using a water purification system (Mili-Q, Millipore, Milford, MA, USA).

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Ostrowska J., Samborowska E., Jaworski M., Toczyłowska K, & Szostak-Węgierek D. (2024). The Potential Role of SCFAs in Modulating Cardiometabolic Risk by Interacting with Adiposity Parameters and Diet. Nutrients, 16(2), 266.

Publication 2024

acetic acid propionic acid butyric acid-BA caproic acid isocaproic acid pyridine anhydrous N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC·HCl)) Mili-Q water Mili-Q

Corresponding Organization : Medical University of Warsaw

Other organizations : Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Children's Memorial Health Institute

Top 5 similar protocols

Variable analysis

independent variables

Concentration of short-chain fatty acid (SCFA) standards
Concentration of isotope-labeled SCFA standards

dependent variables

Measurement of SCFA concentrations

control variables

Solvent (50% acetonitrile) for preparing SCFA stock solutions
Storage temperature (-20 °C) for SCFA stock solutions
LC-MS grade and HPLC-grade solvents (acetonitrile, methanol, water, formic acid) used
Water purification system (Mili-Q) used to produce ultra-pure water

positive controls

Short-chain fatty acid standards (acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid, caproic acid, isocaproic acid)

negative controls

Isotope-labeled SCFA standards (acetic acid-13C2, propionic acid-D6, butyric acid-13C2, isobutyric acid-D7, valeric acid-D9)

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