ERG immunohistochemistry was performed as previously described (42 (link)). Brie y, four μm TMA sections were dehydrated and blocked in 0.6% hydrogen peroxide in methanol for 20 min. and were processed for antigen retrieval in EDTA (pH 9.0) for 30 min in a microwave followed by 30 min of cooling in EDTA buffer. Sections were then blocked in 1% horse serum for 40 min and were incubated with the ERG-MAb mouse monoclonal antibody developed at CPDR (9FY, Biocare Medical Inc.) at a dilution of 1:1280 for 60 min at room temperature. Sections were incubated with the biotinylated horse anti-mouse antibody at a dilution of 1:200 (Vector Laboratories) for 30 min followed by treatment with the ABC Kit (Vector Laboratories) for 30 min. The color was developed by VIP (Vector Laboratories,) treatment for 5 minutes, and the sections were counter stained by hematoxylin. ERG expression was reported as positive or negative. ERG protein expression was correlated with clinico-pathologic features.
Szallasi Z., Diossy M., Tisza V., Li H., Sahgal P., Zhou J., Sztupinszki Z., Young D., Nuosome D., Kuo C., Jiang J., Chen Y., Ebner R., Sesterhenn I., Moncur J., Chesnut G., Petrovics G., T.Klus G., Valcz G., Nuzzo P., Ribli D., Börcsök J., Prósz A., Krzystanek M., Ried T., Szüts D., Rizwan K., Kaochar S., Pathania S., D’Andrea A., Csabai I., Srivastava S., Freedman M., Dobi A, & Spisak S. (2024). Increased frequency of CHD1 deletions in prostate cancers of African American men is associated with rapid disease progression without inducing homologous recombination deficiency. Research Square.
Other organizations :
Boston Children's Museum, Children's Hospital, Danish Cancer Society, HUN-REN Research Centre for Natural Sciences, Institute of Molecular Life Sciences, Prostate Cancer Research, Dana-Farber Cancer Institute, Harvard University, Uniformed Services University of the Health Sciences, Boston Children's Hospital, Boston University, Montavid Thermodynamic Research Group, Eötvös Loránd University, Baylor College of Medicine, University of Massachusetts Boston
ERG protein expression (reported as positive or negative)
control variables
EDTA (pH 9.0) for antigen retrieval
1% horse serum for blocking
ERG-MAb mouse monoclonal antibody (9FY, Biocare Medical Inc.) at 1:1280 dilution
Biotinylated horse anti-mouse antibody at 1:200 dilution (Vector Laboratories)
ABC Kit (Vector Laboratories)
VIP (Vector Laboratories) for color development
Hematoxylin counterstaining
controls
Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned
Annotations
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