Protocol detail

Establishing Cell Culture Conditions

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RPMI-8226, NCI-H929, U266, HEK293T/17 and HS-5 were purchased from ATCC. KMS-11 and MOLP-8 were a kind gift from Professor H. Johnsen (Aarhus University Hospital, Denmark). GFP-tagged bone marrow stromal cells, HS-5-GFP, were generated as previously described [29 (link)]. HEK293T/17 and HS-5-GFP cells were cultured in DMEM containing GlutaMAX™ and 10% FBS (Life Technologies). All other cells were cultured in RPMI-1640 containing GlutaMAX™ and 10% FBS. All cells tested negative for mycoplasma by PCR and were authenticated by STR analysis.

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Fok J.H., Hedayat S., Zhang L., Aronson L.I., Mirabella F., Pawlyn C., Bright M.D., Wardell C.P., Keats J.J., De Billy E., Rye C.S., Chessum N.E., Jones K., Morgan G.J., Eccles S.A., Workman P, & Davies F.E. (2018). HSF1: Essential for Myeloma Cell Survival and A Promising Therapeutic Target. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(10), 2395-2407.

Publication 2018

RPMI-8226 NCI-H929 HEK293T/17 GlutaMAX™ FBS

Bone marrow stromal cells Cells Mycoplasma

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Variable analysis

independent variables

Cell lines (RPMI-8226, NCI-H929, U266, KMS-11, MOLP-8, HEK293T/17, HS-5, HS-5-GFP)

dependent variables

Not explicitly mentioned

control variables

Culture media (DMEM containing GlutaMAX™ and 10% FBS, RPMI-1640 containing GlutaMAX™ and 10% FBS)
Mycoplasma testing (all cells tested negative by PCR)
Cell authentication (all cells authenticated by STR analysis)

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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