Targeting cellular pathways with inhibitors

XL388 was provided by Dr. Zhang [24 (link)] at Southeast University of China. N-acetylcysteine (NAC), pyrrolidine dithiocarbamate (PDTC), AGI-1067, puromycin, rapamycin, perifosine and LY294002 were obtained from Sigma-Aldrich (St. Louis, MO). Cell culture reagents were purchased from Gibco-BRL Co. (Grand Island, NY). Antibodies utilized in this study were provided by Cell Signaling Tech (Shanghai, China) and Abcam Co. (Beijing, China). TRIzol and other reagents for RNA assays as well as Lipofectamine 2000 and other transfection reagents were provided by Thermo-Fisher Invitrogen Co. (Shanghai, China).

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Zhong S., Xue J., Cao J.J., Sun B., Sun Q.F., Bian L.G., Hu L.Y, & Pan S.J. (2020). The therapeutic value of XL388 in human glioma cells. Aging (Albany NY), 12(22), 22550-22563.

Publication 2020

N-acetylcysteine pyrrolidine dithiocarbamate AGI-1067 puromycin rapamycin perifosine LY294002 Cell culture reagents TRIzol Lipofectamine 2000

Agi 1067 Antibodies Assays Cell culture Lipofectamine 2000 Ly294002 N acetylcysteine Perifosine Puromycin Pyrrolidine dithiocarbamate Rapamycin Transfection Trizol Xl388

Corresponding Organization :

Other organizations : Shanghai Jiao Tong University, Ruijin Hospital

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Variable analysis

independent variables

XL388 provided by Dr. Zhang
N-acetylcysteine (NAC)
Pyrrolidine dithiocarbamate (PDTC)
AGI-1067
Puromycin
Rapamycin
Perifosine
LY294002

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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