MCF-7 Cell Line Maintenance and Cytotoxicity

The MCF-7 cell line (human breast cancer epithelial cells) was obtained from the National Centre for Cell Science, Pune, India. For the culture maintenance and in vitro cytotoxicity experiment, MCF-7 cells were cultured in a culture flask or on plates (Falcon, Sigma-Aldrich, Bengaluru, India) in DMEM, supplemented with 20% heat inactivated FBS, 1% penicillin, streptomycin, and amphotericin B in stable conditions of 37 °C and 5% CO₂ in a humidified atmosphere. A passage no higher than 20 was used (P18 in most of the experiments). The cells were frequently tested against mycoplasma according to the laboratory protocol [27 (link)].