IL-6 levels in cell culture supernatant were analyzed using the ELISA MAX™ Deluxe Set Human IL-6 kit (BioLegend, San Diego, CA, USA). After terminating the TMB reaction with stop reagents, the optical density of the reaction mixture was measured at 450 and 570 nm (OD450 and OD570, respectively) within 15 minutes to obtain the experimental result14 (link),22 (link)
24 (link, link)
.