Shrimp Growth and Immune Response

Before rearing shrimp for growth performance analysis, 20 shrimp were randomly selected for proximate analysis of the initial shrimp body. After 70 days of feeding with the treated feed, the shrimp were fasted for 24 hours. Then, the final weight was determined, and three shrimps (per tank) were sampled for proximate body analysis. A total of 3 × 3 shrimp per treatment (three replicates) had hemolymph extracted from the base of the swimming legs using a one mL syringe to create two 0.8 mL tubes (0.4 mL hemolymph and 0.4 mL anticoagulant). The anticoagulant used was Inviclot® Heparin Sodium 5000 IU. The shrimp's hemolymph was used to test its immune response. The shrimp were surgically dissected, and the hepatopancreas was removed to determine the antioxidant activity. Anesthesia (when removing hemolymph and injecting pathogenic bacteria into the shrimp body) used was Clove Bud Oil from PT. Tamba Sanjiwani at a dose of 200 μL L⁻¹ seawater using immersion method. The same quantity of hemolymph and the hepatopancreas was collected from each treatment on the second day (48 hours after bacterial injection) postchallenge test. The initial and final shrimp samples were stored in a -20°C freezer, while hemolymph and the hepatopancreas samples were kept in a -80°C freezer.