Mass Photometry Analysis of NET1-Glycosaminoglycan Complexes
Corresponding Organization : University of Cologne
Other organizations : University of Manitoba, University of Freiburg, University of Oxford, Max Planck Institute for Biology of Ageing, Max Delbrück Center
Variable analysis
- Presence of HO-dp8 or HO-dp10 in the incubation buffer with apo-NET1ΔC or NET1ΔC
- Interaction of the protein with the glass surface, as measured by the intensity of the spot in the interferometric videos
- Protein mass, as calculated from the intensity of the spot in the interferometric videos
- Monomer-dimer or monomer-hexamer ratios for the NET1ΔC-dp8 and NET1ΔC-dp10 complexes, respectively
- Glass slide size (24 × 50 mm #1.5 special, Menzel-Gläser)
- APTES functionalization of the glass slides
- Incubation buffer composition (50 mM tris pH 8.5, 200 mM NaCl)
- Incubation temperature (4 °C)
- Interferometric video acquisition settings (477 nm laser, 1.5 μm focus, 1000 Hz frame rate, 200 Hz final frequency after averaging)
- Alcohol dehydrogenase, β-amylase, and Protein A used for mass calibration
- None explicitly mentioned
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