Schistosomula cultures were daily observed by inverted light microscopy (ABO 100, ZEISS) to verify viability and phenotypic changes, such as movement, color, and tegument integrity.
To evaluate the motility of the worms, we capture worm movement (eight males or females separately) for 1 min and 30 s for 7 days using the WormAssay software (Marcellino et al., 2012 (link)), in six replicates. Additionally, to verify the influence of SmCARM1 on oviposition, eggs laid in the media were counted daily in the cultures containing worm pairs, in five replicates.
To evaluate the motility of the worms, we capture worm movement (eight males or females separately) for 1 min and 30 s for 7 days using the WormAssay software (Marcellino et al., 2012 (link)), in six replicates. Additionally, to verify the influence of SmCARM1 on oviposition, eggs laid in the media were counted daily in the cultures containing worm pairs, in five replicates.
Free full text: Click here
