Ultrastructural Analysis of HCEC-B4G12 Cells

Using a dose of 50 μg/mL, the five types of Mt (Na-Mt, H-Na-Mt, C-H-Na-Mt, Ca-Mt, and MMt) were respectively used to treat HCEC-B4G12 cells for 24 h, after which the cells were seeded in 6-cm-diameter dish and cultured overnight at 37 °C in a 5%-CO₂ incubator. The cells were washed three times with PBS and collected, then fixed overnight with 2.5% glutaraldehyde solution at 4 °C. After undergoing gradient dehydration and embedding, the sample cells were sectioned by ultrathin slicer, stained and dried, and examined with transmission electron microscope (TEM; Hitachi H7650, Japan).