Isolation and Expansion of MSCs

The first sample contained resident SF-MSCs, and the second aspirate sample contained Sm-MSCs, both of which were retrieved and collected as previously described.^{4 (link)}
The aspirated fluid was centrifuged (500 rcf [relative centrifugal force] for 5 minutes), and cells were resuspended in 10 mL of Dulbecco’s modified Eagle medium (DMEM) containing 100 U/mL of penicillin and 100 mg/mL of streptomycin (all from Invitrogen); the SF cells were split according to the experimental design shown in Appendix Figure A1 (available in the online version of this article). For MSC expansion, cells were cultured in StemMACS expansion medium (Miltenyi Biotec), containing penicillin and streptomycin with twice-weekly media changes, and expanded for 3 or 4 passages. Moreover, donor-matched cultures were used in all experiments when they reached passage 3.