CRISPR and TALEN plasmids used were reported in an earlier study (23 (link)). The sgRNA sequence of CASPASE-2 cloned into px330 is TGGTGAGCAACATATCCTCC.
The retroviral plasmids pMaRX-9His-GFP and pMaRX-9His-MCL-1 were described before (34 (link)).
The retroviral expression plasmids pMSCV PIG (Puro IRES GFP empty vector) (plasmid #21654) was purchased from Addgene. The pMSCV PIG-BID and PIG-BID-D60E were cloned into pMSCV PIG by restriction site XhoI/EcoRI. pcDNA3-Casp2-FLAG (plasmid #11811) was purchased from Addgene. The retroviral expression plasmids mpMIG-caspase-2 and mPMIG-caspase-2C320S were cloned into XhoI/EcoRI-digested mpMIG.
The retroviral plasmids pMaRX-9His-GFP and pMaRX-9His-MCL-1 were described before (34 (link)).
The retroviral expression plasmids pMSCV PIG (Puro IRES GFP empty vector) (plasmid #21654) was purchased from Addgene. The pMSCV PIG-BID and PIG-BID-D60E were cloned into pMSCV PIG by restriction site XhoI/EcoRI. pcDNA3-Casp2-FLAG (plasmid #11811) was purchased from Addgene. The retroviral expression plasmids mpMIG-caspase-2 and mPMIG-caspase-2C320S were cloned into XhoI/EcoRI-digested mpMIG.
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