Metabolic Parameters of Mouse Models

Experiments were performed on 12–13-week-old male C57BL/6J (RRID:IMSR_JAX:000664), C57BL/6N (RRID:IMSR_CRL:027), and NMRI mice (RRID:IMSR_CRL:605), all acquired from Charles River. The mice were fed a standard rodent diet Ssniff Rat/mouse – Maintenance (V1534-000) (Ssniff, Soest, Germany) with 9, 24, and 67% of kcal derived from fat, protein, and carbohydrates, respectively, and water ad libitum. They were housed in individually ventilated cages (Allentown LLC, USA) in groups of 1-4 animals per cage at 20-24°C, 45-65% relative humidity, and a 12-hour day-night lighting cycle. Mice were weighed before sacrifice. Following the sacrifice with CO₂ and cervical dislocation, the mouse was placed on its abdomen, and body length was measured from the nose tip to the base of the tail. Glucose was measured within 5 minutes upon sacrifice in the morning between 8 AM and 9 AM from the tail vein using the Accu-Check Aviva glucometer (Roche, Switzerland). During analysis, body mass index (BMI) was calculated as the ratio between body weight and body surface area (g/m²), where body surface area was calculated according to the following formula (52 (link)):