Protocol detail

Ovarian Cancer Cell Lines Cultivation

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The ovarian cancer cell lines, SKOV3ip1 and HeyA8, were obtained from ATCC and were maintained in RPMI 1640 supplemented with 15% fetal bovine serum and 0.1% gentamicin sulfate (Gemini Bioproducts; Calabasas, CA)^{38 (link)}. SKOV3-TR and HeyA8-MDR were kind gifts from Dr. Isaiah Fidler, Department of Cancer Biology, University of Texas M. D. Anderson Cancer Center and Dr. Michael Seiden, Department of Medicine, Massachusetts General Hospital, respectively.

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Wu S.Y., Yang X., Gharpure K.M., Hatakeyama H., Egli M., McGuire M.H., Nagaraja A.S., Miyake T.M., Rupaimoole R., Pecot C.V., Taylor M., Pradeep S., Sierant M., Rodriguez-Aguayo C., Choi H.J., Previs R.A., Armaiz-Pena G.N., Huang L., Martinez C., Hassell T., Ivan C., Sehgal V., Singhania R., Han H.D., Su C., Kim J.H., Dalton H.J., Kowali C., Keyomarsi K., McMillan N.A., Overwijk W.W., Liu J., Lee J.S., Baggerly K.A., Lopez-Berestein G., Ram P.T., Nawrot B, & Sood A.K. (2014). 2’f-OMe-phosphorodithioate modified siRNAs show increased loading into the RISC complex and enhanced anti-tumour activity. Nature communications, 5, 3459.

Publication 2014

SKOV3ip1 HeyA8 RPMI 1640 fetal bovine serum gentamicin sulfate

Cancer Cell lines Fetal bovine serum Gentamicin sulfate Gifts Medicine Ovarian cancer

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Variable analysis

independent variables

Cell lines: SKOV3ip1, HeyA8, SKOV3-TR, HeyA8-MDR

dependent variables

Not explicitly mentioned

control variables

Maintenance media: RPMI 1640 supplemented with 15% fetal bovine serum and 0.1% gentamicin sulfate

controls

Positive control: None mentioned
Negative control: None mentioned

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