Esophageal epithelial cell culture and TGF-β1 treatment
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Corresponding Organization : The Wistar Institute
Other organizations : UPMC Hillman Cancer Center
Protocol cited in 10 other protocols
Variable analysis
- Recombinant human TGF-β1 (5 ng/ml)
- AG 1478 (100 nM)
- Spindle-shaped cell count (at least 100 cells per high-power field, n=6)
- Keratinocyte-serum free medium (Invitrogen, Carlsbad, CA)
- Temperature (37°C)
- Atmosphere (5% CO2)
- Trypan Blue dye (0.2%) to exclude dead cells
- Countess™ Automated Cell Counter (Invitrogen) to count cells
- HCE7, an ESCC cell line
- None specified
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