Estrogen and Progesterone Receptor Evaluation

Clone 6 F11 and clone 1E2 (Ventana Medical Systems, Inc., Tucson, AZ) were used for estrogen and progesterone receptor evaluation, respectively. With the Allred score, the proportion score and the intensity score are assessed in six and four grades, respectively 0-5 and 0-3, then the total score is assessed in eight grades (0 and 2-8) [16 (link)]. Tumors with an immunohistochemistry (IHC) total score of 3 were reported as positive. A score index of 0, 1, 2, and 3 was used, corresponding to negative, weak, moderate, and strong staining intensity, respectively, and the percentage of positive cells at each intensity was estimated subjectively. HER2/neu scores of 0-1 were considered negative, whereas a score of 3 was reported as positive (DAKO). Chromogenic in-situ hybridization or fluorescence in-situ hybridization analyses were performed in cases with IHC total score of 2.