Caco-2 and MDA-MB-231 Cell Lines: Culture and Modification

Human epithelial colorectal adenocarcinoma cell line Caco-2 and human breast cancer cell line MDA-MB-231 were purchased from American Type Culture Collection (ATCC). Cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) supplemented with 10% fetal bovine serum (FBS, Invitrogen), 100 units/mL penicillin, and 100 μg/mL streptomycin (Invitrogen) in a 37 °C incubator containing 5% CO₂. MNPs used in this study were purchased from Beijing Tong Ren Tang Chinese Medicine Co Ltd. SPIO nanodots were synthesized according to our previously reported procedures [11 (link)]. Caco-2 with overexpression of ASBT was generated through lentiviral transduction and selected by puromycin. Lentiviral construct was established by subcloning ASBT cDNA (also called SLC10A2, R&D Systems) into pCDH-MCS-T2A-Puro vector (System Biosciences). Artificial lentiviruses were produced using a second generation of packing systems, including pMD2.G and psPAX2 (Addgene) according to our previous reports [12 (link), 13 (link)]. Other reagents, if not specified, were purchased from Sigma-Aldrich.

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Yang X., Ma C., Chen Z., Liu J., Liu F., Xie R., Zhao H., Deng G., Chen A.T., Gong N., Yao L., Zuo P., Zhi K., Wang J., Gao X., Wang J., Fan L, & Zhou J. (2019). Single small molecule-assembled nanoparticles mediate efficient oral drug delivery. Nano research, 12(10), 2468-2476.

Publication 2019

Caco-2 MDA-MB-231 DMEM FBS penicillin streptomycin pCDH-MCS-T2A-Puro vector pMD2.G psPAX2

Adenocarcinoma Breast cancer cell line Cdna Cell line Cells Chinese Eagle Human Lentiviruses Medicine Penicillin Puromycin Spio Streptomycin Vector

Corresponding Organization :

Other organizations : Harbin Institute of Technology, Beijing Forestry University, Yale University, Beijing Normal University, Chinese Academy of Medical Sciences & Peking Union Medical College

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Variable analysis

independent variables

Overexpression of ASBT in Caco-2 cells
MNPs from Beijing Tong Ren Tang Chinese Medicine Co Ltd
SPIO nanodots synthesized according to previously reported procedures

dependent variables

Not explicitly mentioned

control variables

Caco-2 and MDA-MB-231 cell lines
Culture medium (DMEM) supplemented with 10% FBS, 100 units/mL penicillin, and 100 μg/mL streptomycin
37 °C incubator containing 5% CO2

controls

Positive control: Caco-2 cells
Negative control: Not specified

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