TXNRD1 Genetic Variation Protocol

14 SNPs covering the whole TXNRD1 genetic variability were prioritized by a tagging approach, attempting to choose those most likely to be of functional relevance (nonsynonymous SNPs, SNPs located in the 5′ and 3′ UTR regions). SNPs associated with health status and longevity in Northern Europeans [14 (link), 15 (link)] were also chosen. SNPs with a minor allele frequency (MAF) less than 5% were excluded from the analysis.
Multiplex SNP genotyping was performed using iPLEX Gold Genotyping Assay and Sequenom MassARRAY (Sequenom, San Diego, CA, USA) according to manufacturer's instructions. Sequenom's MassARRAY Designer was used to design PCR and extension primers for each of the 14 SNPs selected. However, four of them (rs10861169, rs10861197, rs10047589, and rs4964287) were skipped by the software for primers design and were not analyzed in this paper. The details for genotyped SNPs are listed in Table 1.