MDSC Characterization by Flow Cytometry

MDSCs were obtained as described above. Cells were incubated with fluorochrome-labeled antibodies at 4°C. Specific antibodies include CD33 PE, HLA-DR PC7, CD11b APC, CD14 Pacific Blue and CD15 FITC (Beckman Coulter, Brea, CA). Flow cytometry was performed as previously described (20 (link)).

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del Campo S.E., Levine K.M., Mundy-Bosse B.L., Grignol V.P., Fairchild E.T., Campbell A.R., Trikha P., Mace T.A., Paul B.K., Jaime-Ramirez A.C., Markowitz J., Kondadasula S.V., Guenterberg K.D., McClory S., Karpa V.I., Pan X., Olencki T.E., Monk J.P., Mortazavi A., Tridandapani S., Lesinski G.B., Byrd J.C., Caligiuri M.A., Shah M.H, & Carson WE I.I.I. (2015). The Raf Kinase Inhibitor Sorafenib Inhibits JAK-STAT Signal Transduction in Human Immune Cells. Journal of immunology (Baltimore, Md. : 1950), 195(5), 1995-2005.

Publication 2015

CD33 PE HLA-DR PC7 CD11b APC CD14 Pacific Blue CD15 FITC

Antibodies Cd11b Cells Fitc Flow cytometry Fluorochrome Hla dr Mdscs

Corresponding Organization : The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute

Other organizations : Nationwide Children's Hospital, Neurological Surgery, The Barbara Ann Karmanos Cancer Institute, Barnes-Jewish Hospital, University of Hawaii System

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Variable analysis

independent variables

Fluorochrome-labeled antibodies (CD33 PE, HLA-DR PC7, CD11b APC, CD14 Pacific Blue, CD15 FITC)

dependent variables

Flow cytometry results

control variables

Cell incubation temperature (4°C)

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

Annotations

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