Quantifying Hippocampal Protein Levels

Levels of protein expression of hippocampal tissue (including whole tissue cell lysate, cytosolic or nuclear fractions) were carried out as previously described [23 (link)]. The optical density of the bands was measured and quantified by Image J (National Institute of Health, MD, USA). Primary antibodies of SOD-2, GPx-1, NFκB p65 and p50, IκBα, TNFα, IL-1β, IL-6 and COX-2 were purchased from Santa Cruz Biotechnology, CA, USA; Synapsin-1 and Synaptophysin were purchased from Novus Biologicals, USA; PSD95 and Cleaved Caspase 3 was purchased from Cell Signaling Technology; Cleaved PARP1 was purchased from Bioworld Technology; IDO-1 was purchased from antibodies-online (ABIN1714836). The data were expressed as percentage of the control.