Cell staining and sorting were performed as previously described (12 (link)). Fluorescence was measured using a BD FACSymphony cell analyzer with FACSDiva version 8.0.1 software. Compensation, gating, and analysis were performed using FlowJo (versions 9 and 10). Cell sorting was performed using a BD FACSAria III cell sorter. Reagents used for flow cytometry are listed in Table 1.
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