Characterization of Tight Junction Protein Antibodies

Mouse anti-ZO-1 monoclonal antibody (mAb) (T8/754), rat anti-occludin mAb (MOC37), and rabbit anti-claudin-2 polyclonal antibody (pAb) were characterized as described previously [41 (link)–43 (link)]. Mouse anti-claudin-2 mAb (32–5600), rabbit anti-claudin-3 pAb (34–1700), alexa fluor 488 phalloidin (A12379) and alexa fluor 647 phalloidin (A22287) were purchased from Life Technologies. Mouse anti-E-cadherin mAb (ECCD-2; M108) was purchased from Clontech. Mouse anti-Na⁺/K⁺ ATPase α1 mAb (NB300-146) was purchased from Novus Biologicals. Fluorescein isothiocyanate-dextran (FITC-dextran), benzamil (B2417), bumetanide (B3023), ouabain (O3125), H89 (B1427), genistein (G6649), Go6983 (G1918) and 3-isobutyl-1-methylxanthine (IBMX, I5879) were purchased from Sigma Aldrich. Fluorescein (16106–82) was purchased from Nacalai tesque. SQ22536 was purchased from Santa Cruz. Forskolin was purchased from Wako.