Sericin-Based Ophthalmic Formulations with Nanoparticles

The 1% sericin solution and nanoparticles (MHN) were prepared using a bead mill method reported by previous study [21 (link),22 (link),23 (link),24 (link)]. The sericin ophthalmic formulations (Sericin/MH fixed combination) containing MHP (P-Ser) or MHN (N-Ser) were prepared by mixing the sericin solution and MH formulations (MHP and MHN). Figure 1 shows the scheme in the preparation process of N-SER. The final concentrations in the P-Ser and N-Ser were as follows: sericin 1%, MH 0.01%, MC 0.5%, BAC 0.005%, mannitol 0.5%, pH8.5, isotonization, sterilized). The size of the MH particles N-Ser was determined by both a laser diffraction particle size analyzer SALD-7100 (Shimadzu Corp., Kyoto, Japan) and dynamic light scattering NANOSIGHT LM10 (Quantum Design Japan, Tokyo, Japan). The Atomic Force Microscope (AFM) images of MHNand N-Ser were provided by an SPM-9700 (Shimadzu Corp., Kyoto, Japan) [21 (link),22 (link)].