Cell Culture Conditions for Various Cell Lines

70Z/3 cells (ATCC, Manassas, VA) were grown in RPMI supplemented with 10% FBS, 0.1 mM glutamax, 10 mM HEPES, and 1 mM sodium pyruvate. 293FT cells (Invitrogen, Carlsbad, CA) were grown in OptiMEM supplemented with 5% FBS. OP9 cells (ATCC, Manassas, VA) were cultured in alpha MEM supplemented with 20% FBS, 1mM sodium pyruvate, and 55uM BME. BCL1, a Balb/c derived B cell lymphoma cell line [11 (link),12 (link)], was cultured in RPMI supplemented with 10% FBS. Primary mouse cells were cocultured on OP9 cells in IMDM supplemented with 10% defined FBS, 55uM BME, 0.1mM Glutamax, 50ng/mL SCF, 10ng/mL IL-3, and 10ng/mL IL-6. Recombinant mouse cytokines were obtained from R&D Systems (Minneapolis, MN) or Invitrogen (Carlsbad, CA). All media contained 50 U/ml penicillin and 50mg/ml streptomycin. 70Z/3 RFP and 70Z/3 RFP Arid3b overexpressing cells were generated as described previously [13 (link)].