Western Blot Analysis of Lipid Metabolism

WB was performed as previously described [29 (link)]. Each experiment was successfully carried out three times, as indicated. The following antibodies were used for WB: anti-HMGCS2 antibody (1:1000, Abcam, Cambridge, MA, USA), anti-ACC antibody (1:1000, Cell Signaling, Beverly, MA, USA), anti-phospho (S79) ACC antibody (1:1000, Cell Signaling, Beverly, MA, USA), anti-FASN antibody (1:1000, Cell Signaling, Beverly, MA, USA), anti-Lipin1 antibody (1:1000, Cell Signaling, Beverly, MA, USA), anti-SREBP2 antibody (1:1000, Cell Signaling, Beverly, MA, USA), anti-ACSL1 antibody (1:1000, Cell Signaling, Beverly, MA, USA), anti-GAPDH antibody (1:2000, Proteintech, Chicago, IL, USA) anti-α-tubulin antibody (1:5000, Sigma-Aldrich, St. Louis, MO, USA). The ImageJ software (National Institutes of Health, Bethesda, MD, USA) was used to quantify the images.