RWPE1 cells were purchased from ATCC ( CRL-11609, Lot 61840713 ) and grown in Keratinocyte Serum Free Medium (Life Technologies ) supplemented with penicillin and streptomycin. Cells were STR profiled by ATCC before purchase, and all experiments were performed on low passage cells (< 10 passages). Lenti6-SCHLAP1 was a kind gift of A. Chinnayian 1 (link). Lenti6-Lacz was generated by removing SCHLAP1 from the above plasmid and cloning LACZ from pLentipuro-LACZ into pLenti6 from which SCHLAP1 was removed by EcoRI-BamHI digest. Lentiviral particles were produced using psPAX2 and pMD2.G to package plasmids in 293T cells. RWPE1;Lacz and RWPE1;SCHLAP1 cells were then generated by transduction with lentivirus and selecting with 2.5ug/mL blasticidin for 1 week. 22RV1 and LNCaP Cells were purchased from ATCC (CRL-2505, Lot 60437301; CRL-1740, Lot 62129998) and grown in RPMI 1640 with 10% Fetal Bovine Serum supplemented with penicillin and streptomycin. Cells were STR profiled by ATCC prior to purchase and were used at early passages ( < 10 passages). All cells were tested and free of mycoplasma contamination.